Rabu, 24 Oktober 2012

Mikroskop VIVAVIEW FL INCUBATOR FLUORESCENCe

 

VIVAVIEW FL INCUBATOR FLUORESCENCE MICROSCOPE OVERVIEW

  • Ideal time-lapse imaging inside an incubator
  • Parallel time-lapse observation of eight dishes
  • Eliminates thermal drift during long-term imaging
  • High quality fluorescence and DIC observation
  • Cooled CCD camera
  • Stress-free time-lapse imaging
  • Simple computer based control
  • Powerful and proven image analysis with MetaMorph for Olympus
  • Ability to load chemical solutions during growth

VIVAVIEW FL INCUBATOR FLOURESCENCE MICROSCOPE SPECIFICATIONS

Objective UPLSAPO40x (NA 0.95, WD 0.18mm)
Intermediate magnifications 0.5x/1x/2x, Motorized magnification changer
Morphological observation (Transmitted light) Light source 1W LED (625nm)
Contrast method Differential Interference Contrast (DIC)
Condenser NA 0.55, FS fixed (rectangle), DIC prism fixed
Fluorescence Observation (incident light) Light Source X-Cite eXacte Pre-aligned 2000 hour DC lamp with Closed - Loop Feedback, Adjustable Iris, Liquid Light Guide
Excitation change Motorized 6 position, 1 for transmitted light
Motorized Stage Stroke 10mm x10mm, each dish
Sample Capacity Glass bottom dishes with 35mm diameter, up to 8
Sample Tray Can be easily put on and taken off, autoclave capability, include 8 lids with glass window for the glass bottom dishes with 35mm diameter.
Focus
Objective drive, motorized up/down control
Operation Jog dial control
Camera Cooled CCD camera, -35oC air cooled
2/3" (8.67mm x 6.60mm), 1344 x 1024 pixels
12 bits or 16 bits
Liquid handling assist (BTO option) Motorized open/close control of designated dish. Manual pipetting through the small opening on the internal door of the incubator.
Imaging software MetaMorph
CO2Incubator Temperature 37°C
Accuracy +/- 0.3°C (ambient temperature 25oC, no load)
Humidity 95% +/- 5% RH
CO2Concentration 0-20%
Accuracy +/- 0.15% (ambient temperature 25°C, no load)
Operating conditions Temperature 15°-28°C
Humidity Less than 80% RH
Dimensions of main body 710mm x 630mm x 1170mm
Weight 150kg
Electricity power consumption Main body 500VA
PC and monitor About 400VA


Aplikasinya

VIVAVIEW FL INCUBATOR FLUORESCENCE MICROSCOPE APPLICATIONS

Click Here to view VivaView FL Video Gallery

PUBLICATIONS

  • Meeting the Challenges of Long-term Time-lapse Imaging - Bioscience Technology Sept 2009 (PDF) Read More >
  • Takahiro Seki, Takayuki Shimahara, Kazuhiro Yamamoto, Nana Abe, Taku Amano, Naoko Adachi, Hideyuki Takahashi, Kaori Kashiwagi, Naoaki Saito, Norio Sakai. (2009). Mutant ?PKC found in spinocerebellar ataxia type 14 induces aggregate-independent maldevelopment of dendrites in primary cultured Purkinje cells. Neurobiology of Disease. Vol. 33, 260-273. Read More>
  • Asako Sakaue-Sawano, Hiroshi Kurokawa, Toshifumi Morimura, Aki Hanyu, Hiroshi Hama, Hatsuki Osawa, Saori Kashiwagi, Kiyoko Fukami, Takaki Miyata, Hiroyuki Miyoshi, Takeshi Imamura, Masaharu Ogawa, Hisao Masai, and Atsushi Miyawaki. (2008). Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression. Cell. Vol. 132, 487-498. Read More>
  • Asako Sakaue-Sawano, Kenji Ohtawa, Hiroshi Hama, Masako Kawano, Masaharu Ogawa, and Atsushi Miyawaki. (2008). Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology. Vol. 15, 1243-1248. Read More>
  • Hiroki Umeshima, Tomoo Hirano, and Mineko Kengaku (2007). Microtubule-based nuclear movement occurs independently of centrosome positioning in migrating neurons.
    PNAS. Vol.104, No. 41, 16182-16187. Read More>
  • Takahiro Hatanaka, Yasue Hatanaka, Jun-ichi Tsuchida, Vadivel Ganapathy, and Mitsutoshi Setou. (2006) Amino Acid Transporter ATA2 Is Stored at the trans-Golgi Network and Released by Insulin Stimulus in Adipocytes. Journal of Biological Chemistry. Vol. 281, No. 51, 39273-39284. Read More>

VIDEOS

Screening drugs to block aggregation of Huntingtin protein

Apoptosis: An award-winning video

Instrument: VivaView FL (LCV-110)
Subject: This video is a series of live, time-lapse images of human mesenchymal stem cells grown on a collagen-I coated dish. The cells are treated with a high level of a PI3-Kinase inhibitor which induces apoptosis (or programmed cell death). This video won an award at the 2010 American Society for Cell Biology Cell Dance competition.
Reference: Video courtesy of Ms. Kira Henderson, PhD student, Department of Biology, Rennselaer Polytechnic Institute, Troy, NY.
PLAY VIDEO
Screening drugs to block aggregation of Huntingtin protein

Screening drugs to block aggregation of Huntingtin protein

Instrument: VivaView FL (LCV-110)
Subject: Montage displaying aggregate accumulation of GFP-tagged truncated Huntingtin in PC12 cells under 3 different concentrations of an aggregation inhibitor drug vs. control media.
Reference: Emily Mitchell, Leslie Thompson, Ph.D., University of California, Irvine
PLAY VIDEO
Human neural stem cells are healthy during two weeks of imaging

Human neural stem cells are healthy during two weeks of imaging

Instrument: VivaView FL (LCV-110)
Subject: 2 week time lapse of Human Neural Stem cells transfected with GFP CAG. Development is followed after the addition of differentiation media at T = 24 hours.
Reference: Chris Sontag, Brian J. Cummings, Ph.D., University of California, Irvine
PLAY VIDEO
Comparing effects of immunosuppressant drugs on human neural stem cell fate

Comparing effects of immunosuppressant drugs on human neural stem cell fate

Instrument: VivaView FL (LCV-110)
Subject: Immunosuppressant drug study of Human Neural Stem cells to analyze proliferation and migration. Movie shows a 2 x 2 montage from a single specimen dish. Focus is maintained throughout as new drug and media is added every 3 days.
Reference: Chris Sontag, Brian J. Cummings, Ph.D., University of California, Irvine
PLAY VIDEO
Migration tracking of human neural stem cells

Migration tracking of human neural stem cells

Instrument: VivaView FL (LCV-110)
Subject: Tracking data of Human Neural Stem cells captured at 15 frames per second.
Reference: Chris Sontag, Brian J. Cummings, Ph.D., University of California, Irvine
PLAY VIDEO

Biocompare Video for VivaView FL Incubator Fluorescence Microscope

PLAY VIDEO

Hela Cells constantly-expressing Fucci

Instrument: VivaView FL (LCV-110)
Subject: VivaView FL makes a contribution as the microscope to provide a stable environment in culture for the long term. 64 hours 13.5 minutes.
Reference: Sakaue-Sawano A, Atsushi Miyawaki M.D., Ph.D. RIKEN Brain Science Institute, Laboratory for Cell Function Dynamics
PLAY VIDEO

HeLa Cells expressing Histone H2B-GFP Cells divide 3 times during 60 hours of fluorescence and DIC observation

Instrument: VivaView FL (LCV-110)
Subject: Successful observation of 3 divisions in cells expressing GFP localized to the nucleus is challenging for fluorescence microscopy. The VivaView FL allows long-term imaging by minimizing damage to the cells while maintaining image qality.
Reference: Atsushi Miyawaki M.D., Ph.D. RIKEN Brain Science Institute, Laboratory for Cell Function Dynamics
PLAY VIDEO

Purkinje neuron

Instrument: VivaView FL (LCV-110)
Subject: Fluorescence time lapse observation of primary cell cultures is extremely difficult. Using the VivaView FL, Dr. Kengaku successfully captures a 4 days time-lapse data series of a neuron.
Reference: Mineko Kengaku Ph.D., RIKEN Brain Science Institute, Laboratory for Neural Cell Polarity
PLAY VIDEO

Superior cervical ganglion cells

Instrument: VivaView FL (LCV-110)
Subject: Transportation of Mitochondria in neurites. The VivaView FL captures mitochondrial movement that is faster than previous reported.
Reference: Mitsutoshi Setou, M.D., Ph.D., Molecular Gerontology, Mitsubishi Kagaku Institute of Life Sciences.
PLAY VIDEO

PC12 cell with GFP - Model system for neuronal differentiation

Instrument: VivaView FL (LCV-110)
Subject: 6 daytime-lapse data captured at 2 hour intervals. The VivaView FL maintains cell viability for over 6 days of imaging.
PLAY VIDEO

KAMI PT. FAJARMASMURNI AGEN RESMI (TUNGGAL) MIKROSKOP OLYMPUS JAPAN WILAYAH INDONESIA KALAU BERMINAT DAN INGIN TAU TENTANG OLYMPUS HUBUNG SAYA 
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EMAIL :  agustiar@fajarmasmurni.com
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